Background:  The genus Enterococcus includes more than 17 species and until 1984, Enterococcus species were classified as Group D Streptococcus. This genus belongs to lactic acid bacteria of the phylum Firmicutes. Enterococci are Grampositive cocci, they are facultative anaerobic organisms that can survive and grow in many environments. They are part of the normal intestinal flora of humans and animals but also found in soil, water, plants and insects. Enterococci are important pathogens responsible for serious infections. In cattle, enterococci have been associated with diarrhea in calves and bovine mastitis in dairy cattle.

Description:  BactoReal® Kit Enterococcus spp. uses real-time PCR for the detection of the 23S rRNA gene of species of the genus Enterococcus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Enterococcus spp.
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB03411 100 FAM VIC 23S rRNA
DVEB03413 100 FAM Cy5 23S rRNA
DVEB03451 50 FAM VIC 23S rRNA
DVEB03453 50 FAM Cy5 23S rRNA
For research use only. Not for use in diagnostic procedures.
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 23S rRNA gene of Enterococcus spp.
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Enterococcus spp. is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Kit Enterococcus spp.
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV905 100 FAM 23S rRNA
For research use only. Not for use in diagnostic procedures.
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.