Download
| Product Manual BactoReal® Kit Histophilus somni |
Download PDF
|
| Material Safety Data Sheet BactoReal® Kit Histophilus somni |
Download PDF
|
Download All
|
Background: Histophilus somni is an unencapsulated gram-negative bacterium and a member of the Pasteurellaceae family. It is a common commensal in the lower reproductive and upper respiratory tract of cattle and other ruminants. H. somni is one of the bacterial agents responsible for bovine respiratory disease (BRD) complex and associated with respiratory disease, arthritis, mastitis, myocarditis and reproductive failure in sheep and cattle. Other important bacterial pathogens of BRD are Pasteurella multocida, Mannheimia haemolytica, Trueperella pyogenes and Mycoplasma bovis. Mixed infections with these organisms do occur.
Description: BactoReal® Kit Histophilus somni uses real-time PCR for the detection of the 16S rRNA gene of Histophilus somni. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DVEB06113 | 100 | FAM | Cy5 | 16S rRNA |
| DVEB06153 | 50 | FAM | Cy5 | 16S rRNA |
PCR platforms: BactoReal® Kit Histophilus somni is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| RTGV333 | 100 | FAM | Cy5 | 16S rRNA |