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Product Manual BactoReal® Kit Lawsonia intracellularis Download PDF
Material Safety Data Sheet BactoReal® Kit Lawsonia intracellularis Download PDF
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Background:  Lawsonia intracellularis is a Gram-negative and obligate intracellular bacterium and the causative agent of porcine proliferative enteropathy (PPE), which is a transmissible enteric disease of growing pigs characterized by adenomatous proliferation of immature intestinal epithelial cells in the distal small intestines, particularly in the ileum. The infection is widely distributed among pig herds in many pig-producing countries, and affected pigs may exhibit impaired growth and diarrhoea.

Description:  BactoReal® Kit Lawsonia intracellularis uses real-time PCR for the detection of the 16S rRNA gene of L. intracellularis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Lawsonia intracellularis
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB01313 100 FAM Cy5 16S rRNA
DVEB01353 50 FAM Cy5 16S rRNA
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 16S rRNA gene of Lawsonia intracellularis
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Lawsonia intracellularis is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Lawsonia intracellularis
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV300 100 FAM Cy5 16S rRNA
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.