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Product Manual ViroReal® Lumpy Skin Disease Virus Download PDF
Material Safety Data Sheet ViroReal® Lumpy Skin Disease Virus Download PDF
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Background:  Lumpy skin disease virus (LSDV) is a dsDNA virus within the genus Capripoxvirus. It is genetically very similar to the other two Capripoxvirus species sheeppox virus and goatpox virus. There is one serotype of LSDV. LSDV causes lumpy skin disease in cattle and buffalo. It is transmitted by arthropod vector. Traditionally it has been found in southern Africa but has been steadily spreading north to the Middle East and into eastern Europe and Russia.

Description:  ViroReal® Kit Lumpy Skin Disease Virus uses RT real-time PCR for the GPCR gene of the lumpy skin disease virus.. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

ViroReal® Lumpy Skin Disease Virus
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV03313 100 FAM Cy5 GPCR
DVEV03353 50 FAM Cy5 GPCR
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: GPCR gene of Lumpy Skin Disease Virus
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit Lumpy Skin Disease Virus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® Lumpy Skin Disease Virus
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV34 100 FAM Cy5 GPCR
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.