Background: Mycoplasma hyopneumoniae is a primary pathogen of respiratory disease in pigs. This bacterium is one of the most important causative agents of the enzootic pneumonia (EP) and of the porcine respiratory disease complex (PRDC).

Description: BactoReal® Kit Mycoplasma hyopneumoniae uses real-time PCR for the detection of the rpoB gene of M. hyopneumoniae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.

BactoReal® Kit Mycoplasma hyopneumoniae
OrdernumberReactionsChannel PathogenChannel IPCTarget
DVEB00111100FAMVIC/HEXrpoB
DVEB00113100FAMCY5rpoB
DVEB0015150FAMVIC/HEXrpoB
DVEB0015350FAMCY5rpoB
For veterinary use only.
Shipping Temperature: -20°C dry ice
Product Features:
  • Amplification and detection: rpoB gene of Mycoplasma hyopneumoniae
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Mycoplasma hyopneumoniae is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.


Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Mycoplasma hyopneumoniae
OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGV210100FAM-rpoB
For veterinary use only.
Shipping Temperature: +4°C cool packs

Related Products: BactoReal®Mycoplasma hyopneumoniae Standard

BactoReal®, MycoReal, ParoReal and ViroReal® Kits run with the same thermal cycling conditions.