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Product Manual BactoReal® Kit Mycoplasma hyopneumoniae Download PDF
Material Safety Data Sheet BactoReal® Kit Mycoplasma hyopneumoniae Download PDF
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Background:  Mycoplasma hyopneumoniae is a primary pathogen of respiratory disease in pigs. This bacterium is one of the most important causative agents of the enzootic pneumonia (EP) and of the porcine respiratory disease complex (PRDC).

Description:  BactoReal® Kit Mycoplasma hyopneumoniae uses real-time PCR for the detection of the rpoB gene of M. hyopneumoniae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Mycoplasma hyopneumoniae
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB00113 100 FAM Cy5 rpoB
DVEB00153 50 FAM Cy5 rpoB
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: rpoB gene of Mycoplasma hyopneumoniae
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Mycoplasma hyopneumoniae is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Mycoplasma hyopneumoniae
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV210 100 FAM Cy5 rpoB
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.