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Product Manual BactoReal® Kit Pasteurella multocida toxA Download PDF
Material Safety Data Sheet BactoReal® Kit Pasteurella multocida toxA Download PDF
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Background:  Pasteurella multocida is a Gram-negative bacterium and part of the flora in the respiratory tract of pigs. The bacterium induces hemorrhagic septicemia or, usually as a secondary pathogen invading lungs injured by other bacteria or viruses, pneumonia. A subset of P. multocida isolates are critical agents in an upper respiratory disease, progressive atrophic rhinitis (PAR). Pasteurella multocida strains associated with PAR usually produce a dermonecrotic toxin, encoded by the toxA gene. The toxA protein of these isolates acts as an essential virulence factor inducing turbinate atrophy and poor weight gain in pigs. Non-toxigenic P. multocida strains are not usually associated with PAR. Therefore, confirmation of toxin production is important for the diagnosis and control of the disease.

Description:  BactoReal® Kit Pasteurella multocida uses real-time PCR for the detection of the toxA gene of P. multocida. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Pasteurella multocida toxA
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB00913 100 FAM Cy5 toxA
DVEB00953 50 FAM Cy5 toxA
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: toxA gene of Pasteurella multocida
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Pasteurella multocida is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Pasteurella multocida toxA
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV400 100 FAM Cy5 toxA
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.