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| Product Manual BactoReal® Kit Streptococcus uberis |
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| Material Safety Data Sheet BactoReal® Kit Streptococcus uberis |
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Background: Streptococcus uberis is a Grampositive bacterium known worldwide as an environmental pathogen responsible for a high proportion of cases of clinically acute as well as subclinical bovine mastitis.
Description: BactoReal® Kit Streptococcus uberis uses real-time PCR for the detection of ITS region of S. uberis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DVEB01613 | 100 | FAM | Cy5 | ITS region |
| DVEB01653 | 50 | FAM | Cy5 | ITS region |
- Amplification and detection: ITS region of Streptococcus uberis
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or Assay-only
PCR platforms: BactoReal® Kit Streptococcus uberis is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| RTGV700 | 100 | FAM | Cy5 | ITS region |