Background: Trichinellosis is a food-borne parasitic zoonosis caused by nematodes of the genus Trichinella. Twelve genotypes of Trichinella have been identified worldwide, four of which are confirmed to exist in Europe: T. spiralis, T. nativa, T. britovi and T. pseudospiralis. Two main clades are recognized in the genus: one group (T. britovi, T. murrelli, T. nativa, T. nelsoni, T. spiralis) that encapsulates in host muscle tissue and a second (T. papuae, T. pseudospiralis, T. zimbabwensisi) that does not.

Description: ParoReal Kit Trichinella spiralis & britovi uses real-time PCR for the detection of the internal transcribed spacer of Trichinella spiralis and T. britovi. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.

ParoReal Kit Trichinella spiralis & britovi
OrdernumberReactionsChannel PathogenChannel IPCTarget
DVEP00413100FAM & VIC/HEXCY5internal transcribed spacer
DVEP0045350FAM & VIC/HEXCY5internal transcribed spacer
For veterinary use only.
Shipping Temperature: -20°C dry ice
Product Features:
  • Amplification and detection: internal transcribed spacer of Trichinella spiralis & T. britovi
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ParoReal Kit Trichinella spiralis & britovi is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.


Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ParoReal Trichinella spiralis & britovi
OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGV268100FAM & VIC/HEX-internal transcribed spacer
For veterinary use only.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal, ParoReal and ViroReal® Kits run with the same thermal cycling conditions.