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Product Manual ViroReal® Maedi-Visna Virus Download PDF
Material Safety Data Sheet ViroReal® Maedi-Visna Virus Download PDF
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Background:  The Maedi-Visna virus (MVV) also called ovine lentivirus (OvLV) is a single-stranded RNA small ruminant lentivirus (SRLV) within the retrovirus family. There are neurovirulent and non-neurovirulent strains of MVV. The MVV is closely related to the caprine arthritis - encephalitis (CAE) virus. Both infect cells of the monocyte/macrophage lineage and cause lifelong infections. Visna (“wasting”, in Icelandic) is a slow progressive encephalomyelitis of sheep. Although most infections are subclinical, a minority of animals develops progressive, untreatable disease syndromes including dyspnea (maedi), slowly progressive arthritis, mastitis or neurologic signs (visna) such as encephalitis.

Description:  ViroReal® Kit Maedi-Visna virus uses one-step reverse transcription RT real-time PCR for the detection of the gag polyprotein gene of Maedi-Visna virus (MVV). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

ViroReal® Kit Maedi-Visna Virus
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV03513 100 FAM Cy5 gag polyprotein gene
DVEV03553 50 FAM Cy5 gag polyprotein gene
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: gag gene of Maedi-Visna virus
  • Real-time PCRwith primern, probes and positive control
  • PCR- platforms: runs on all established standard real-time PCR- platforms

PCR platforms: ViroReal® Kit Maedi-Visna virus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® Maedi-Visna Virus
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV36 100 FAM Cy5 gag polyprotein gene
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.