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Product Manual BactoReal® American Foulbrood | Download PDF |
Material Safety Data Sheet BactoReal® American Foulbrood | Download PDF |
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Background: American foulbrood (AFB) is a bacterial brood disease of honeybees caused by the Grampositive and spore-forming bacterium Paenibacillus larvae. The disease is transferred and initiated only by the spore stage of the bacterium. Spores infect young bee larvae, then the spores germinate in the gut of the larva and the vegetative form of the bacteria begins to grow, taking its nourishment from the larva. Infected larvae normally die after their cell is sealed. The vegetative form of the bacterium produces many millions of spores, which may remain dormant for many years.
Description: BactoReal® Kit American Foulbrood 1.1 uses real-time PCR for the detection of the 16S rRNA gene of Paenibacillus larvae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
---|---|---|---|---|
DVEB05413 | 100 | FAM | Cy5 | 16S rRNA |
DVEB05453 | 50 | FAM | Cy5 | 16S rRNA |
- Amplification and detection: 16S rRNA gene of Paenibacillus larvae
- Real-time PCRwith primern, probes and positive control
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms: BactoReal® Kit American Foulbrood 1.1 is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
---|---|---|---|---|
RTGV924 | 100 | FAM | Cy5 | 16S rRNA |