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| Product Manual ViroReal® MERS-CoV |
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| Material Safety Data Sheet ViroReal® MERS-CoV |
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Background: Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a beta coronavirus causing Middle East Respiratory Syndrome (MERS), a zoonotic and severe respiratory disease in humans. To date, MERS-CoV has only been isolated from dromedary camels and humans. In humans, it was first identified in April 2012. Camels are a likely primary source of the MERS-CoV infection in humans.
Description: ViroReal® Kit MERS-CoV uses one-step reverse transcription RT real-time PCR for the detection of the nucleocapsid (N) gene of Middle East Respiratory Syndrome Coronavirus (MERS-CoV). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DVEV03013 | 100 | FAM | Cy5 | nucleocapsid (N) gene |
| DVEV03053 | 50 | FAM | Cy5 | nucleocapsid (N) gene |
- Amplification and detection: N gene of MERS-CoV
- Real-time PCRwith primern, probes and positive control
- PCR- platforms: runs on all established standard real-time PCR- platforms
PCR platforms: ViroReal® Kit MERS-CoV is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| RTGV31 | 100 | FAM | Cy5 | nucleocapsid (N) gene |