MycoReal Fungi

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Background: Fungal diseases are mostly opportunistic infections with a great variety of common and uncommon pathogens. Up to 150 fungal species have been shown as potential human pathogens involving all body sites. Invasive mycoses are increasingly recognized as a primary cause of morbidity and mortality especially in immunocompromised patients. The early diagnosis improves prognosis. Invasive mycoses are mainly caused by yeasts and moulds. Non-invasive fungal infections (such as infections of the urogenitary tracts, of the eye, of the skin, etc.) are mainly caused by Candida, Aspergillus, Acremonium, Fusarium and dermatophytes.

Description: MycoReal Fungi is a panfungal real-time PCR detection assay for rapid and sensitive broad-range detection of fungal DNA. This assay has the potential to detect microorganisms found less frequently as well as uncultivable or even unknown causative agents of fungal origin such as yeasts, moulds, zygomycetes, dermatophytes, endemic fungi and others. Fungal DNA is detected via amplification curves. For identification of the species (only possible for samples containing a single fungal species), the generated panfungal PCR-product has to be sequenced and phylogenetically analysed. Since MycoReal Assays (MycoReal Aspergillus, MycoReal Candida, MycoReal Pneumocystis and MycoReal Fungi) use identical temperature/time profiles, they can be combined in the same LightCycler® run. This allows the detection of a wide panel of pathogenic fungi within only a few hours.

PCR platforms: MycoReal Fungi is developed and validated for the LightCycler® 2.0 instrument (Roche).

Assay: This product is available as an assay-only format with primers, probe and a positive control, but without reaction mix.