Background: Bartonella species are Gramnegative bacteria. In addition to dogs and cats, numerous domestic and wild animals, including bovine, canine, and rodent species as well as humans can serve as a reservoir host for various Bartonella species. Cats may harbor B. henselae, B. clarridgeiae and rarely other Bartonella species. Dogs may carry Bartonella vinsonii subsp. berkhoffii, Bartonella henselae and others.

Description: BactoReal® Kit Bartonella spp. uses real-time PCR for the detection of the gltA gene of Bartonella species. This kit is specific for B. clarridgeiae, B. elizabethae, B. grahamii, B. henselae, B. koehlerae, B. quintana, B. volans and B. washoensis. Bartonella rochalimae, B. vinsonii and B. bovis are detected with very low sensitivity. Bartonella bacilliformis is not detected. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.

BactoReal® Kit Bartonella spp.
OrdernumberReactionsChannel PathogenChannel IPCTarget
DVEB03911100FAMVIC/HEXgltA gene
DVEB03913100FAMCY5gltA gene
DVEB0395150FAMVIC/HEXgltA gene
DVEB0395350FAMCY5gltA gene
For veterinary use only.
Shipping Temperature: -20°C dry ice
Product Features:
  • Amplification and detection: gltA gene of Bartonella spp.
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Bartonella spp. is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.


Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Bartonella spp.
OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGV1260100FAM-gltA gene
For veterinary use only.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal, ParoReal and ViroReal® Kits run with the same thermal cycling conditions.