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Product Manual BactoReal® Kit Borrelia burgdorferi sensu lato Download PDF
Material Safety Data Sheet BactoReal® Kit Borrelia burgdorferi sensu lato Download PDF
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Background:  Borrelia are Gram-negative bacteria belonging to the Spirochaetaceae. Borrelia burgdorferi sensu stricto, B. garinii, B. afzelii, B. valaisiana, B. lusitaniae and B. spielmanii belong to the B. burgdorferi sensu lato-complex and cause Lyme disease (Lyme borreliosis). These species are associated with different genospecies specific symptoms. They are transmissed by ticks, mainly by Ixodes ricinus. Lyme disease is a multisystemic illness, which can affect various organs, especially the nervous system, skin, heart and joints.

Description:  BactoReal® Kit Borrelia burgdorferi sensu lato uses real-time PCR for the detection of the ospA gene of Borrelia species belonging to the Borrelia burgdorferi sensu lato complex. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG) to minimize PCR cross-contamination.

BactoReal® Kit Borrelia burgdorferi sensu lato
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB05013 100 FAM Cy5 ospA gene
DVEB05053 50 FAM Cy5 ospA gene
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: ospA gene of Borrelia burgdorferi sensu lato
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Borrelia burgdorferi sensu lato is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Borrelia burgdorferi sensu lato
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV1270 100 FAM Cy5 ospA gene
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.