Background: Brachyspira hyodysenteriae (previously called Serpulina hyodysenteriae or Treponema hyodysenteriae) is an anaerobic intestinal spirochaete and the causative agent of swine dysentery. Swine dysentery is a disease characterized by mucohaemorrhagic diarrhoea with lesions confined to the large intestine of pigs. Its incubation time is 10-14 days. The faecal excretion of B. hyodysenteriae starts 2 days after infection. Brachyspira hyodysenteriae is an anaerobic bacterium but is aerotolerant due, at least in part, to high NADH oxidase activity.

Description: BactoReal® Kit Brachyspira hyodysenteriae uses real-time PCR for the detection of the NADH oxidase (nox) gene of B. hyodysenteriae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG) to minimize PCR cross-contamination.For more detailed information see here.

BactoReal® Kit Brachyspira hyodysenteriae
OrdernumberReactionsChannel PathogenChannel IPCTarget
DVEB01111100FAMVIC/HEXnox
DVEB01113100FAMCY5nox
DVEB0115150FAMVIC/HEXnox
DVEB0115350FAMCY5nox
For veterinary use only.
Shipping Temperature: -20°C dry ice
Product Features:
  • Amplification and detection: nox gene of Brachyspira hyodysenteriae
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Brachyspira hyodysenteriae is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.


Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Brachyspira hyodysenteriae
OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGV610100FAM-nox
For veterinary use only.
Shipping Temperature: +4°C cool packs

Related Products: BactoReal®Brachyspira hyodysenteriae Standard

BactoReal®, MycoReal, ParoReal and ViroReal® Kits run with the same thermal cycling conditions.