Background: Swine Influenza is an acute, febrile respiratory disease of swine with high morbidity and low mortality. In the industry it is commonly known as Swine Flu, Hog Flu, and Pig Flu. It is caused by the influenza virus, mainly influenza type A, an enveloped virus with single-stranded, segmented RNA with negative polarity as genome. Influenza A virus infects a wide variety of warm-blooded animals including pigs, birds, horses as well as humans.

Description: ViroReal® Kit SIV uses one-step reverse transcription RT real-time PCR for the detection of the matrix protein gene of the influenza A virus of warm-blooded animals including pigs, birds, horses as well as humans. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well. For more detailed information see here.

ViroReal® Kit Swine Influenza A (SIV)
OrdernumberReactionsChannel PathogenChannel IPCTarget
DVEV00411100FAMVIC/HEXmatrix protein gene
DVEV00413100FAMCY5matrix protein gene
DVEV0045150FAMVIC/HEXmatrix protein gene
DVEV0045350FAMCY5matrix protein gene
For veterinary use only.
Shipping Temperature: -20°C dry ice
Product Features:
  • Amplification and detection: matrix protein gene of Swine Influenza A
  • Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
  • contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit SIV is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.


Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® Swine Influenza A (SIV)
OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGV15100FAM-matrix protein gene
For veterinary use only.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal, ParoReal and ViroReal® Kits run with the same thermal cycling conditions.