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Product Manual ViroReal® KitSwine Influenza A (SIV) Download PDF
Material Safety Data Sheet ViroReal® KitSwine Influenza A (SIV) Download PDF
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Background:  Swine Influenza is an acute, febrile respiratory disease of swine with high morbidity and low mortality. In the industry it is commonly known as Swine Flu, Hog Flu, and Pig Flu. It is caused by the influenza virus, mainly influenza type A, an enveloped virus with single-stranded, segmented RNA with negative polarity as genome. Influenza A virus infects a wide variety of warm-blooded animals including pigs, birds, horses as well as humans.

Description:  ViroReal® Kit SIV uses one-step reverse transcription RT real-time PCR for the detection of the matrix protein gene of the influenza A virus of warm-blooded animals including pigs, birds, horses as well as humans. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

ViroReal® KitSwine Influenza A (SIV)
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV00413 100 FAM Cy5 matrix protein gene
DVEV00453 50 FAM Cy5 matrix protein gene
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: matrix protein gene of Swine Influenza A
  • Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
  • contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit SIV is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® Swine Influenza A (SIV)
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV15 100 FAM Cy5 matrix protein gene
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.